Chapter 10

What is the process of tranferring “naked” DNA fragments from a donor cell to competent recipient cell
Transformation
Beside the gene of interest, most vector plasmids also contain what feature ?
Antibiotic resistance gene
We will write a custom essay sample on
Chapter 10
or any similar topic only for you
Order now
Containing media is used to locate recombinant cloning host in a culture
Antibiotic
Put the 3 basic steps of PCR in order
1-denaturation
2-priming
3-extension
The process —–/—– is portrayed in the image seen here
Gel electrophoresis
What is the molecule that serve as the master plan for an organism’s characteristics and heredity
DNA
The —- of a DNA fragment determines the distance it moves during gel electrophoresis
Size
What enzyme synthesis the new strand of DNA in PCR?
DNA polymerase
The— of nucleic acids are denoted by the number of base pairs they contain
Size
Large DNA fragments move farther than smaller fragments in the gel when electrophoresis is used
True
—–/—–is the enzyme that can convert RNA into DNA
Reverse transcriptase
Is a laboratory technique for separating DNA fragments according to length by employing electricity to force DNA through a gel-like matrix
Gel electrophoresis
What is the enzyme responsible for unwinding DNA
Helicase
Genomic analysis can be helpful in which of the following situations
-determining the paternity of a child
-detecting genes that indicate risk of disease
-identifying the perpetrator of a crime
Restriction enzymes recognize and clip at sequences called promoters
Palindromes are sequence locations where restriction enzymes cut
False
Some diseases are actually due to inappropriate expression or over expression of a
Protein
Put these general steps in order for the recombination DNA procedure
1-DNA of interest is isolated or produce from mRNA
2- DNA is inserted into vector
3- Microbial cloning host is transformed when it pick up vector with foreign DNA
In the laboratory DNA maintains its normal properties
True
Is the amount of DNA in a sample over a short of time
PCR amplifies
Which of the following is used in the laboratory to separate strands of DNA
Heat
Technology involving the analysis of —- ca be used to differentiate between members of the same species
DNA
Identify the molecule that is being synthesized in the image shown here
rDNA
Which of the fallowing make a good cloning vector
Plasmids and bacteriophages
DNA that is made from RNA is called —- DNA
Complementary
Gene therapy involves the introduction of normal fictional genes into people with genetic disease such as sickle cell anemia and cystic fibrosis
True why
Which strategy or strategies can be used to isolate a target gene for use in recombinant DNA technology
1-Digestion with endonuclease
2-synthesis of cDNA
3-amplification by PCR
The main role of restriction endonuclease is to protect against foreign DNA in the biotechnology lab
False
RNA can hybridize with —–or—-
DNA or RNA
Gel electrophoresis provides a distinctive pattern that can be used to compare genetic similarities between individuals
True
If a condition is due to the appropriate expression of a gene, a solution would be to inhibit which process in the cell
Translation
Genetic engineering provides the possibility of one day fixing the mutual — responsible for certain disease
Genes
What is the first challenge in the recombinant DNA process in cloning a target gene
Locating and isolating a target gene
Stranggered cuts through the two strands of DNA by an endonuclease leave tails known as—-ends
Sticky
The pieces of DNA produced by restriction endonuclease are termed restriction fragments which
True
The process of — is used to introduce a recombinant plasmid to the cloning host
Transformation
Genes probes are commonly use for
– identifying bacteria or viruses in a unknown culture
-diagnosing causative agents of infection
Sequencing is a process in which complementary single strands of nucleic acids bind to one another
False
The process of—is used to introduce a recombinant plasmid to the cloning host
Transformation
What 3 qualities must a cloning vector possess
-must have a promoter in front of the gene
-must be capable of carrying donor DNA
-Must be accepted by cloning host
A palindrome is a sequence that is identical both forwards and backwards
True
DNA that has been separated into its 2 strands when the— is increased is called heat-denatured DNA
Temperature
What is the process of producing a genetic identical population of cells or genes in recombinant DNA technology
Cloning
Which of the following describes the advantages of creating transgenic animals
-animals that can make products useful to humans
-similarity between animals and humans
-animal models to test new therapies
-animal models to study human disease
DNA — involves determining the exact order of nucleotides in a fragment of DNA
Sequencing
The new fields is— and bioinformatics have arisen to ultimately determine the function of genes being sequenced
Genomics
The –/–/–is a technique that makes numerous copies of DNA segments for testing
Polymerase chain reaction
Cloning host commonly used in recombinant DNA technology include
Live animals and plants
Yeast
Animal cell cultures
Several bacterial species
Several hundred strains of transgenic animals have been created
True
Place the following in order to indicate the naming process for restriction enzymes
First letter of bacterial genus
First 2 letters of bacterial species
The order discovered in the bacterium
The bacterium—-/— is the most commonly used cloning host
Echerichia coli
A genetic element such as a plasmid or a bacteriophage that is used to introduce genetic material into a cloning host during recombinationa DNA experiments is called a vector
True
Recombinant organisms produced trough the introduction of foreign genes are called—– or genetically modified organisms (GMOs)
Transgenic
Which type of chemical bonds in DNA are broken by restriction endonuclease
Phosphodiester
The organism that receives and replicates a foreign piece of DNA inserted during a genetic engineering experiment is referred to as the
Cloning host
What enzyme cleaves specific location on DNA and is used to splice genes in genetic engineering
Endonuclease
—- involves separating the two strands of target DNA during PCR
Desnaturacion
What is the primary intent of recombinant DNA technology
Combine genetic material from two organisms
–and— of the foreign gene to make the protein product will occur if a cloning host receives the recombinant plasmidp
Transcription /translation
Each PCR cycle — the number of copies of DNA
Doubles
DNA fingerprinting is also known as DNA typing or profiling
Fingerprinting
×

Hi there, would you like to get such a paper? How about receiving a customized one? Check it out