DNA-Based Information Technologies

Restriction enzymes:
are sequence-specific DNA endonucleases
The biological role of restriction enzymes is to:
degrade foreign DNA that enters a bacterium
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The size of the DNA region specifically recognized by type II restriction enzymes is typically:
4 to 6 base pairs
Which of the following statements about type II restriction enzymes is false?
they cleave and ligate DNA
Certain restriction enzymes produce cohesive (sticky) ends.. This means that they:
make staggered double-strand cut, leaving ends with a few nucleotides of single-stranded DNA protruding
In the laboratory, recombinant plasmids are commonly introduced into bacterial cells by:
transformation – heat shock of the cells incubated with plasmid DNA in the presence of CaCl2
The E. coli recombinant plasmid pBR322 has been widely utilized in genetic engineering experiments. pBR322 has all of the following features EXCEPT:
a number of palindromic sequences near the EcoRI site, which permit the plasmid to assume a conformation that protects newly inserted DNA from nuclease degradation
Which of the following statements regarding plasmid cloning vectors is correct?
the copy number of plasmids may vary from a few to several hundred
A convenient cloning vector with which to introduce foreign DNA into E. coli is a(n):
plasmid
In genetic engineering, in vitro packaging is used to:
incorporate recombinant DNA into infectious bacteriophage particles
Which of the following does NOT apply to the construction or use of a DNA library?
genomic libraries are better for expressing gene products than cDNA libraries
The PCR reaction mixture does NOT include:
DNA ligase
Which of the following statements about the polymerase chain reaction (PCR) is FALSE?
DNA is amplified at many points within a cellular genome
RFLP is a:
variation in DNA base sequence
Current estimates indicate that humans have about _________ genes.
30,000
Which one of the following analytic techniques does NOT help illuminate a gene’s cellular function?
Southern blotting
The technique known as two hybrid analysis for detecting interacting gene products depend on:
stimulation of transcription by interaction of two Gal4p domains via fused protein sequences
A common cloning strategy for introducing foreign genes into plants with Agrobacterium employs all the following features EXCEPT:
a shuttle vector with 25 bp T-DNA repeats flanking the foreign gene of choice
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