Question Answer
eyepiece ocular lens magnifies 10x
body tube carries filtered light from objective lens to ocular lens
coarse adjustment knob generally focuses specimen
fine adjustment knob precisely focuses specimen
nosepiece holds objective lenses, adjustable
objectives variable magnifying lenses
stage clip holds slides in place, adjusts location
arm connects magnifying lenses to base of microscope
stage location of specimen
mechanical stage holds slide/ specimen in place, allows for slight rearrangement of location
iris diaphragm allows increase or decrease of light through condenser
condenser concentrates light rays on specimen
inclination joint point of arm adjustment (bends)
pillar connects base to stage
base foot of microscope
mirror reflects lights through condenser
sub stage adjustment lever adjust height of condenser
objective low power 10x
high power 45x
oil immersion 97x
total magnification magnification of ocular X magnification of objective lens
low power=
high power=
oil immersion=
10x X 10x= 100x
45x X 10x= 450x
97x X 10x= 970x
transport 1. firmly grasp the curved arm of the microscope
2. lift microscope up
3. place the other hand under there base of the microscope and carry to desired location.
storage 1. raise body tube & remove slide
2. condenser down
3. clean off objectives w/ lens tissue
4. place 10x objective over stage opening
5. lower body tube w/ course adjustment knob (all the way down) place cover over microscope
parfocal microscopes are constructed so that changing from a lower to a higher magnification requires only an adjustment w/ the fine adjustment knob. always focus on 10x first before going on to 45x or 97x. locate the area slide u wish to examineunder low power
place microscope 8-14 inches away from light source
raise condenser all the way up
use two hands to adjust the concave side of the mirror so the brightest possible light comes through the glass top of the condenser
lower condenser half a turn
looking from the side lower the 10x objective close to the slide w/o touching
to focus look into the eyepiece and turn course adjustment knob toward yourself slowly
how to bring into clear focus the fine adjustment knob
after focusing on 10x swing 45x objective into place
final focus 45x w/ adjustment knob
oil immersion raise condenser all the way up
turn nosepiece so objective is moved to the side
place 1 drop of oil on the slide where light is shining through the stage
turn nosepiece so 97x is in the oil
focus w/ fine adjustment only

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