| Question | Answer |
|---|---|
| eyepiece | ocular lens magnifies 10x |
| body tube | carries filtered light from objective lens to ocular lens |
| coarse adjustment knob | generally focuses specimen |
| fine adjustment knob | precisely focuses specimen |
| nosepiece | holds objective lenses, adjustable |
| objectives | variable magnifying lenses |
| stage clip | holds slides in place, adjusts location |
| arm | connects magnifying lenses to base of microscope |
| stage | location of specimen |
| mechanical stage | holds slide/ specimen in place, allows for slight rearrangement of location |
| iris diaphragm | allows increase or decrease of light through condenser |
| condenser | concentrates light rays on specimen |
| inclination joint | point of arm adjustment (bends) |
| pillar | connects base to stage |
| base | foot of microscope |
| mirror | reflects lights through condenser |
| sub stage adjustment lever | adjust height of condenser |
| objective | low power 10x high power 45x oil immersion 97x |
| total magnification | magnification of ocular X magnification of objective lens |
| low power= high power= oil immersion= |
10x X 10x= 100x 45x X 10x= 450x 97x X 10x= 970x |
| transport | 1. firmly grasp the curved arm of the microscope 2. lift microscope up 3. place the other hand under there base of the microscope and carry to desired location. |
| storage | 1. raise body tube & remove slide 2. condenser down 3. clean off objectives w/ lens tissue 4. place 10x objective over stage opening 5. lower body tube w/ course adjustment knob (all the way down) place cover over microscope |
| parfocal | microscopes are constructed so that changing from a lower to a higher magnification requires only an adjustment w/ the fine adjustment knob. always focus on 10x first before going on to 45x or 97x. locate the area slide u wish to examineunder low power |
| place microscope | 8-14 inches away from light source |
| raise condenser | all the way up |
| use two hands to | adjust the concave side of the mirror so the brightest possible light comes through the glass top of the condenser |
| lower condenser | half a turn |
| looking from the side | lower the 10x objective close to the slide w/o touching |
| to focus | look into the eyepiece and turn course adjustment knob toward yourself slowly |
| how to bring into clear focus | the fine adjustment knob |
| after focusing on 10x | swing 45x objective into place |
| final focus 45x | w/ adjustment knob |
| oil immersion | raise condenser all the way up turn nosepiece so objective is moved to the side place 1 drop of oil on the slide where light is shining through the stage turn nosepiece so 97x is in the oil focus w/ fine adjustment only |
