Lab 3 Flagellate protozoa

*Chilomastix mesnili *
– Host = human
– NH= colon
– I. S= cyst
– D. S= cyst + Trophozoite
– specimen = stool sample
– transmission = contaminated food or water with cyst

– Chilomastix mesnili
– cyst

D.F
1- Lemon in shape with anterior small nipple like projection.
2- Large single nucleus with large central karyosome.

*Giardia lamblia*
– Host = human & other mammals
– N.H= small intestine
– I.S = cyst
– D.S = cyst + Trophozoite
– specimen = stool sample
– transmission =
1- contaminated food or water with cyst
2- fecal oral route

– Giardia lamblia
– cyst

D.F
1- 4 nuclei with central karyosome, all located at one end.
2- Double cyst wall & cytoplasm retracted from the wall creating aclear zone.

– Giardia lamblia
– trophozoite

D.F
1- Pear or tear drop in shape with 4 pairs of flagella.
2- 2 nuclei with central karyosome,each one situated in adhesive disc.

*Trichosmonas vaginalis*
– Host = human
– N.H= vagina in female & genital tract of male
– specimen =
1- woman: swab collected from vaginal secretion
2- men: swab collected from urethral secretion

– Trichosmonas vaginalis
– trophozoite

D.F
1- Ovoid or pear like in shape with 3-5 flagella.
2- Single ovoid nucleus at the anterior end with short undulating membrane.