Identify bacteria that contain a recombinant plasmid
X-Gal is included in the growth medium on which cells transformed with bacterial plasmids are grown. The reason X-GAL is included is to
True
Within a six base DNA sequence, an enzyme that cuts between the 3rd and 4th bases from the 5′ end will generate blunt ends.
They provide a defense mechanism against infection by viruses
What is the function of restriction endonucleases in bacteria
False
Restriction endonucleases cut DNA at specific recognition sequences and then bond two strands covalently with the same sticky ends
3′ TCTTAAG 5′
BamHI cuts the sequence 5′ G|GATCC 3′. Which of the following sequences would not be recognized by this enzyme?
Palindromic
Words such as did, mom, and pop have something in common compared to the fundamental tool of recombinant DNA technology. In the context of recombinant DNA technology, which term would be used to describe such words?
single stranded complementary tails
Restriction endonucleases are especially useful if they generate sticky ends. What makes an end sticky?
Multiple cloning site
Some vectors such as puc18 and others of the puc series contain a large number of restriction enzyme sites clustered in one region. Which term is given to this advantageous arrangement of restriction sites?
true
A common term for a plasmid or other DNA element that serves as a cloning vehicle is a vector
False
To isolate a bacterium with a plasmid that carries a desired DNA fragment cloned within the ampicillin resistance gene, we should grow bacteria in a medium that contains ampicillin
They protect the bacteria’s DNA by being able to recognize viral DNA and then digesting it.
What might be a reasonable function of restriction endonucleases in a bacterium, distinct from their use by molecular biologists?
To visualize probe hybridization events
Why are filters overlaid with the X-ray film when screening a cDNA library
False
Chromosome walking is used mainly to isolate a gene when the sequence of the gene is known.
to define the target region and provide a 3′ end that can be extended by taq polymerase
The role of primers in PCR is ___
40
If there are five molecules of DNA containing the target region at the beginning of a PCR reaction, how many copies of the target will be present after three rounds of amplification?
the temperature is raised so that taq polymerase can extend the primers
Immediately after the primers have annealed to the target sequence__
True
Phage λ can carry larger DNA fragments than plasmids.
lambda arms
Which of the following elements is not found in plasmid
True
In a PCR, primers are complementary to stretches of DNA with which they anneal
True
The products of restriction digestion can be visualized by gel electrophoresis, which separates fragments based on their size
They have a hydrogen at the 3′ carbon of the sugar
Which of the following statements about ddNTPs is true?
The DNA sequence obtained is complementary to the template strand
Which of the following statements about manual Sanger sequencing is true?
OH; 2′; 3′
A ddNTP, used often in DNA sequencing, lacks a __ at the __ and __carbons
Probes are used to identify and/or locate a particular nucleic acid sequence among a pool of sequences
In context of recombinant DNA technology, of what use is a probe?
