cut the DNA again with restriction enzyme Y and insert these fragments into the plasmid cut with the same enzyme.
Assume that you are trying to insert a gene into a plasmid. Someone gives you a preparation of genomic DNA that has been cut with restriction enzyme X. The gene you wish to insert has sites on both ends for cutting by restriction enzyme Y. You have a plasmid with a single site for Y, but not for X. Your strategy should be to
by adding methyl groups to adenines and cytosines
How does a bacterial cell protect its own DNA from restriction enzymes?
I. Transform bacteria with a recombinant DNA molecule.
II. Cut the plasmid DNA using restriction enzymes.
III. Extract plasmid DNA from bacterial cells.
IV. Hydrogen-bond the plasmid DNA to nonplasmid DNA fragments.
V. Use ligase to seal plasmid DNA to nonplasmid DNA.
III, II, IV, V, I
II. Cut the plasmid DNA using restriction enzymes.
III. Extract plasmid DNA from bacterial cells.
IV. Hydrogen-bond the plasmid DNA to nonplasmid DNA fragments.
V. Use ligase to seal plasmid DNA to nonplasmid DNA.
III, II, IV, V, I
What is the most logical sequence of steps for splicing foreign DNA into a plasmid and inserting the plasmid into a bacterium?
bacteria cannot remove eukaryotic introns.
A principal problem with inserting an unmodified mammalian gene into a BAC, and then getting that gene expressed in bacteria, is that
reverse transcriptase to reconstruct the gene from its mRNA.
A gene that contains introns can be made shorter (but remain functional) for genetic engineering purposes by using
They are eukaryotic cells.
Why are yeast cells frequently used as hosts for cloning?
BACs.
The DNA fragments making up a genomic library are generally contained in
centromeres, telomeres, and an origin of replication
Yeast artificial chromosomes contain which of the following elements?
1. The primers hybridize to the target DNA.
2. The mixture is heated to a high temperature to denature the double-stranded target DNA.
4. DNA polymerase extends the primers to make a copy of the target DNA.
2, 4, 1
2. The mixture is heated to a high temperature to denature the double-stranded target DNA.
4. DNA polymerase extends the primers to make a copy of the target DNA.
2, 4, 1
Which of the following best describes the complete sequence of steps occurring during every cycle of PCR?
a YAC with appropriate cellular enzymes
A researcher needs to clone a sequence of part of a eukaryotic genome in order to express the sequence and to modify the polypeptide product. She would be able to satisfy these requirements by using which of the following vectors?
a BAC
A student wishes to clone a sequence of DNA of ~200 kb. Which vector would be appropriate?
include a transgenic rice plant that can help prevent vitamin A deficiency.
Genetically engineered plants
Microorganisms used in recombinant DNA experiments are genetically crippled to ensure that they cannot survive outside of the laboratory.
Scientists developed a set of guidelines to address the safety of DNA technology. Which of the following is one of the adopted safety measures?
